What Is The Purpose Of Freeze-Thaw Cycles. The purpose of a defrost cycle. The added benefit of multiple cycles occurs in the periphery of the tumor, where the depth of hypothermia is unpredictable and the cell kill unreliable.

The concentrations of iron or other potentially damaging metal ions and their concomitant free radicals can locally spike in trapped aqueous pockets during the freezing and thawing process, exposing nearby nucleic acid strands to damage. The added benefit of multiple cycles occurs in the periphery of the tumor, where the depth of hypothermia is unpredictable and the cell kill unreliable. As the ice expands, it pushes the crack apart, making it larger.